Journal: Cancer Gene Therapy

Article Title: Mitochondrial Fus1/Tusc2 and cellular Ca2 + homeostasis: tumor suppressor, anti-inflammatory and anti-aging implications

doi: 10.1038/s41417-022-00434-9

Figure Lengend Snippet: Systemic, cellular and molecular manifestations of Fus1 decrease/loss/increase in normal and tumor tissues.

Article Snippet: Anaplastic thyroid cancer cell line 8505 C and papillary thyroid cancer cell line TPC-1 (Fus1 level is decreased) , Overexpression of FUS1/TUSC2 increases levels of Smac/Diablo, suppressor of IAPs blocking caspase- and cytochrome c- mediated apoptosis , [ ] .

Techniques: Over Expression, Expressing, Migration, In Vitro, In Vivo, Activation Assay, Blocking Assay, Mass Spectrometry, Plasmid Preparation, Irradiation, Infection, Cell Stimulation, Membrane, Injection, Mouse Assay, Control

Journal: Genes

Article Title: Identification and Validation of a Prognostic Signature for Thyroid Cancer Based on Ferroptosis-Related Genes

doi: 10.3390/genes13060997

Figure Lengend Snippet: Reducing AKR1C3 expression in thyroid cancer cells inhibits proliferation, migration and invasion: ( A ) TPC-1 and 8505c cell lines were transfected with siNC or siAKR1C3, and the level of PHLPP1 mRNA expression was assessed by RT-qPCR; ( B , C ) the CCK-8 assays indicated that knockdown of AKR1C3 inhibits cells proliferation in TPC-1 and 8505c; ( D – G ) the wound-healing and transwell asssays suggested that AKR1C3 reduces cell migration and invasion in TPC-1 and 8505c (** p < 0.01, *** p < 0.001).

Article Snippet: The human anaplastic thyroid cancer cell line 8505c and human papillary thyroid cancer cell line TPC-1 were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany).

Techniques: Expressing, Migration, Transfection, Quantitative RT-PCR, CCK-8 Assay, Knockdown